Poly morphisms of Adefg Efflux Pump Gene' s in Acinetobacter. baumannii isolated from burn sample

A. baumannii is a multidrug-resistant opportunistic pathogen responsible for a range of human diseases. Much of this resistance is due to its efflux pump systems, the AdeFGH system composed of the AdeF, AdeG, and AdeH genes. This study focused on the adeG gene, which encodes the major transport protein of the AdeFGH system, with the aim of assessing the potential role of this gene in antibiotic resistance in burn-derived A. baumannii isolates. For this purpose, sixty bacterial samples were collected from burn patients at multiple hospitals in Baghdad. Biochemical methods were used to identify the bacteria, and disk diffusion was used to determine antibiotic resistance and sensitivity. The adeG gene was then identified by polymerase chain reaction. Resistance to amoxicillin was found to be 95% and to ciprofloxacin to 85%. The lowest resistance was found to imipenem, at 37%. The study aimed to clarify the relationship between the efflux pump resistance gene (adeG) and antibiotic resistance in bacteria. It was found that 70% (42/60) of A. baumannii isolates carried the adeG efflux pump gene. The results indicated sensitivity to antibiotics, and all strains exhibited varying degrees of resistance, with the highest resistance rate to amikacin (95%), tetracycline (65%), and ciprofloxacin (30%). In an analysis of the resistance of isolates containing the AdeG gene, resistance rates were lower, specifically 26.1% to ciprofloxacin, 42.8% to tetracycline, and 30.9% to amikacin.