A cloud-enabled digital twin platform for upstream processing in biotechnology: Integrating CFD, compartmentalization, and reaction kinetics

Biotechnology process modeling has become an essential tool in both research and industrial settings, offering a cost-effective and efficient alternative to extensive physical experimentation. It enables the simulation and analysis of complex biological systems, supporting faster development cycles and better decision-making. To foster collaboration and knowledge sharing in this domain, we present an open source cloud-enabled platform for upstream biotechnology process modeling. The platform provides an integrated environment where users can combine computational fluid dynamics (CFD), compartmental models, and kinetic simulations within a unified and modular interface. Each component of the model operates independently, but can be seamlessly coupled through a standardized API. The system is designed to support both research and educational use cases, with an emphasis on accessibility, extensibility, and reproducibility. This paper outlines the platform architecture and implementation, highlights the technical challenges addressed in model integration, and discusses opportunities for future development. By lowering technical barriers and encouraging community-driven innovation, the platform aims to advance digital twin applications in biotechnology upstream processing. • Open source web-based platform developed for upstream biotechnology process modeling and simulation. • Platform integrates CFD results, compartmental models, and kinetic simulations with a unified interface. • Modular API structure enables easy extension and independent use of model components. • Automated testing frameworks and validation ensure robust performance across platform components. • Platform serves both research and educational purposes and supports collaboration and model sharing.