Thermal responses of Baltic Sea Ostreococcus vary in their levels of intra- and interspecific variability

These are the R Scripts and data files needed to run the statistics in Thermal responses of Baltic Sea Ostreococcus vary in their levels of intra- and interspecific variability Authors: Carina Peters*, Marie Vogel, Luisa Listmann, C.-Elisa Schaum *Corresponding Author: [email protected] There is always a csv file and xlsx file of each dataset, the xlsx file contains explanations for each column. The csv file was used for analysis.The Scripts are for the following analyses: 1. R Script: Growth_Curve_FittingDatasets: Growth_Curve_Fitting_CellCounts.csv, SampleDeath_Acclimation.csv Used for fitting of strain specific growth curves for each experimental temperature and replicate. The dataset Growth_Curve_Fitting_CellCounts.csv contains the cytometric cell counts collected over the period of the experiment. The dataset SampleDeath_Acclimation.csv contains a list of strains that died during acclimation to add a growth rate of zero for those temperatures to the dataset for better fitting of thermal peformance curves in the following script. From the fitted curves we extracted µmax and calcualted day of µmax for fitting of GAMMs in the fourth script. 2. R Script: rTPC_ModelFitting_AveragingDataset: Mumax_perStrain_withzeros.csvThis script cointains the model selection and fitting process of the thermal performance curves (TPCs) per strain, as well as extraction of the TPC parameters. It uses the dataset of µmax generated in the first script. 3. R Script: rTPCparameters_linearmodelDataset: rTPCparameters_averagedThis code contains the linear (and non-parametric) models fitted for the TPC parameters per species. The dataset contains the parameters extracted from the model fits in the second R Script. 4. R Script: GAMM_Chla_Size_atmumax_perSpecies Dataset: GAMM_Chla_Size_atmumax.csvUsed for fitting species specific GAMM response curves across temperatures for Chlorophyll a conent at µmax, size at µmax and µmax. The dataset contains the size and chlorophyll a cytometric measurements for the day that was caluclated to correspond to the day µmax was reached. File: Submicron_Reference_Bead_Standard Curve_Calibration formula.xlsxThis file contains the bead measurements of the sub mircron particle kit used to make a standard curve to convert FSC-H measurements to size in µm.It also contains the formula derived from the fit that was used for conversion in the dataset used in the GAMM analysis.