Variation in Seed Protein Contains of Safflower by SDS- Page

Abstract The protein is an essential part of human diet. It plays an important role in human nutrition. So, the present study was conducted to investigate the variation in seed protein content in healthy and infected seeds of safflower. Two varieties of safflower i.e. var Sharda and var PBNS-12.was under taken to investigate the variation in seed protein content in healthy and seeds. sodium Dodecyl sulfate polyacrylamide Gel electrophoresis (SDS-PAGE ) method was adopted. The band in I and II lane belongs to variety PBNS-12 healthy and diseased seeds. The bonds were compared with standard protein molecular weight marker in lane III. The protein content of var PBNS-12 both in healthy and disease seed ranges between 83.8 KD to 119 KD. Similar results obtained in variety Sharda in healthy and disease seeds. The molecular masses ranged from 26.2KD to 216KD. The significant difference in seed protein was recorded in seed protein content in healthy and infected seed of both varieties. In variety PBNS-12 the significant difference in seed protein was occurred in region 26.2 t 32. 7 KD and 199 KD to 216 KD. While rest of other regions shows similar banding patterns in both the varieties of healthy and infected seeds.