Efficiency of a Double-Phase Medium in Micropropagation of Serviceberry (Amelanchier sp.)

The use of double-phase medium (2F) gave beneficial effects in the propagation of woody plants belonging to the Rosaceae family. Despite this, it appears that such research has not yet been carried out in relation to (Amelanchier sp.). Thus, the efficiency of such a technique in micropropagation of three cultivars of serviceberry (Amelanchier sp.)—‘Autumn Brilliance’, ‘Ballerina’, ‘Snowcloud’—was evaluated. The 2F medium was obtained by pouring the liquid MS solution (10 mL) onto the solid (1F) medium (50 mL) after inoculation of the explants. Generally, the response of the in vitro cultures to 2F medium was positive but clone-dependent. This medium stimulated, to various extents, the elongation and proliferation of the shoots. The use of 2F medium did not significantly increase hyperhydricity, whereas it lowered shoot tip necrosis frequency during the multiplication stage. However, the residual effect of 2F medium on the in vitro rooting of shoots and acclimation was adverse in the case of two out of three studied clones. Considering the efficiency of the three micropropagation stages, the use of 2F medium was only favorable in the case of one clone (‘Ballerina’), yielding over 90% more acclimated plantlets in comparison to the control (1F medium).