Enhanced antibacterial and wound healing properties for Ni-Zn-Co ferrite nanoparticles

The antimicrobial and wound healing properties of Zn 0.2 Co 0.8-x Ni x Fe 2 O 4 (x = 0.2, 0.4, 0.6, and 0.8) nanoparticles, prepared through the sol-gel auto-combustion technique, were examined in this investigation. The physical and chemical characteristics of the obtained materials were studied using XRD, FTIR, XPS, FESEM, EDS, BET, and VSM. When tested for antibacterial action against both Gram-positive and Gram-negative bacteria, the nanoparticles exhibited inhibition against both types of bacteria within the 10 mg/mL dosage range. No significant antifungal activity was observed. The PANC1 and L929 cell line was used in the cytotoxicity test for the synthesized nanoparticles. Fibroblast migration and proliferation were confirmed using a wound healing assay, which involves artificial wound shrinking. For all biological experiments, Zn 0.2 Co 0.8-x Ni x Fe 2 O 4 (x = 0.8) exhibited better responses than other Zn 0.2 Co 0.8-x Ni x Fe 2 O 4 concentrations (x = 0.2, 0.4, and 0.6). These findings demonstrate that employing a combination of doping on the synthesized nanoparticles enhances their biological activity.