The effect of high-intensity interval training and spirulina supplementation on the levels of Pannexin-1 and NLRP-1 proteins in hippocampal tissue in male rats with type 1 diabetes

The purpose of this research is to investigate the effect of High-intensity interval training and spirulina supplementation on the levels of Pannexin-1 and NLRP-1 proteins in hippocampal tissue in male rats with type 1 diabetes. In this experimental study, 30 Wistar rats, aged 10 weeks and weighing 180-260 grams, were randomly divided into 5 groups of 6 each: exercise group, supplement group, exercise + supplement group, control group, 5 healthy group. After inducing diabetes and implementing the protocol, hippocampal tissue from the rats was extracted, and the levels of PANNXIN-1 and NLRP-1 proteins were measured using the western blot method. Statistical analyses and data processing were conducted using SPSS version 26 software, with significance set at P≤0.05. The results showed a significant increase in Pannexin-1 and NLRP-1 proteins in diabetic rats (P<0.05). There was a noticeable decrease in protein expression after 6 weeks of rigorous intermittent exercise and spirulina supplements in the exercise, supplement, and exercise + supplement groups compared to the diabetic group. This reduction was mostly due to the combined effects of exercise and supplementation rather than either alone. Our research has verified the hypoglycemic effects of intense interval training and spirulina supplementation, along with their impact on reducing PANX-1 and NLRP-1 protein expression. Consequently, it appears that this form of physical activity and herbal supplement could significantly enhance the management of neurodegenerative disorders in individuals with type 1 diabetes.