Time-lapse imaging of C. elegans egg-laying with high spatiotemporal resolution

Egg-laying behavior in <i>C. elegans</i> is driven by a simple neural circuit comprising motor neurons, vulva muscles, and neuroendocrine cells. Most protocols designed to capture this behavior rely on imaging freely moving animals at low magnifications, thus limiting subcellular-level understanding of the underlying neuromuscular circuit. Here, we present a protocol to immobilize <i>C. elegans</i> while maintaining egg-laying activity, enabling high spatiotemporal-resolution imaging of this behavior.