The effect of moderate-intensity interval training on plasma subfatin levels and body composition in sedentary obese middle-aged women

Obesity is a major risk factor for metabolic diseases. Subfatin, an adipokine with potential metabolic benefits, is of increasing interest. This study investigated the effects of eight weeks of Moderate-Intensity Interval Training (MIIT) on plasma subfatin levels and body composition in sedentary, obese, middle-aged women. Thirty sedentary women (aged 50-60 years; BMI >30 kg/m²) were purposively selected and randomly assigned to an experimental (n=15) or a control (n=15) group. The experimental group performed an eight-week MIIT program (three sessions/week) at an intensity of 50-75% of heart rate reserve, following the principle of progressive overload. The control group maintained their usual sedentary routine. Fasting plasma subfatin levels were measured via enzyme-linked immunosorbent assay (ELISA), and body composition indices (BMI, body fat percentage [BFP], waist-to-hip ratio [WHR]) were assessed pre- and post-intervention. The MIIT group exhibited a significant increase in plasma subfatin levels (p=0.016) and a significant decrease in BFP (p=0.01) compared to the control group. While positive trends were observed, no significant inter-group differences were found for BMI (p>0.05) or WHR (p=0.095). An eight-week MIIT program effectively elevated plasma subfatin concentration and reduced body fat percentage in sedentary, obese, middle-aged women. These results suggest that MIIT may be a valuable exercise strategy for improving adipokine profile and body composition in this population.