The furosemide spectrum: Development of a precise analytical method using UV spectroscopy

A UV-Visible spectrophotometer was used to measure the absorbance of standard solutions, and linearity was established within a specified range, exhibiting a high correlation coefficient (R² > 0.999), indicating excellent linearity.The absorption maxima method was found to be 277nm. The results confirmed that the method is suitable for routine quality control of Furosemide API in bulk and pharmaceutical formulations. The proposed method is advantageous due to its simplicity, rapidity, and minimal use of reagents and solvents. The study's goal is to develop and validate a straightforward, accurate, and economical UV spectrophotometric method for the quantitative analysis of Furosemide.Furosemide contains conjugated double bonds and aromatic rings (e.g., a substituted benzene ring and sulfonamide group), which are chromophores. These absorb UV light due to π→π* and n→π* electronic transitions, making UV spectroscopy ideal for detecting them.The method were validated with respect to linearity,precision,and accuracy studies.