Role of crude enzymes and microbial consortia in the degradation of organic matter

The rapid expansion of agricultural and industrial sectors has resulted in the accumulation of lipid- and polysaccharide-rich wastes, posing substantial environmental challenges. Microbial biodegradation employing crude enzymes presents a sustainable and eco-friendly approach to mitigating these pollutants.In this study, soil and sludge samples collected from sugarcane and mustard cultivation fields, along with associated processing units, were enriched in selective media to isolate potent enzyme-producing microorganisms. The isolates were screened for lipase, amylase, and cellulase activity using tributyrin agar, carboxymethyl cellulose (CMC) agar, and starch agar, respectively. Enzymatic activity was quantified through titrimetric and DNS assays, while MALDI-TOF analysis confirmed the taxonomic identities. Lipase purification was carried out using ammonium sulfate precipitation, followed by immobilization in calcium alginate beads to assess stability and application potential. A microbial consortium was subsequently developed to evaluate synergistic degradation of organic waste.Seven bacterial isolates belonging to Pseudomonas, Bacillus, Enterococcus, Micrococcus, and Providencia genera exhibited significant enzymatic activity. Lipase activity ranged between 476–588 U/ml, with purification enhancing it up to 1064 U/ml. Although immobilization slightly reduced activity, microbial consortia demonstrated enhanced biodegradation efficiency. Notably, consortia 1 and 4 achieved the highest waste reduction (~11 g over 45 days).These findings highlight the biotechnological potential of enzyme-producing microbes and their consortia in sustainable waste degradation, offering promising applications in industrial effluent treatment and agricultural residue management.