Gas Chromatography-Mass spectrometry (GC-MS) Revealed Bioactive Constituents of Brassica oleracea var. viridis (Collard Greens) Used to Treat Male Infertility in Uganda.

<ns3:p> Background Medicinal plants play a crucial role in pharmacological research, as many pharmaceutical companies rely on them for raw materials. Collard greens, also known as “Sukuma wiki,” are a member of the <ns3:italic>Brassica oleracea</ns3:italic> var. viridis family and are known for their medicinal properties. The aim of this study was to identify phytochemicals in the ethanol extract of collard green leaves that has traditional use in treating male infertility in Uganda. Method The leaves were dried and crushed into fine powder and the powdered leaves (800 g) A was cold macerated in 5 liters of 99% ethanol (1:5 w/v) for 72 hours in sterile glass container. The ethanol extract (5g) was subjected to Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Results Phytochemical analysis of the Ethanol extract from <ns3:italic>Brassica oleracea</ns3:italic> var. viridis (Collard green) leaves revealed the presence of various compounds, including flavonoids, alkaloids, phenolic compounds, fatty acids, and terpenoids. Gas Chromatography-Mass Spectrometry (GC-MS) analysis identified 77 bioactive compounds, such as 2-Methoxy-4-vinylphenol, 2, 7-Dimethyl-1, 7-octadien-3-amine, Octadecanoic acid, 9, 12, 15-Octadecatrienoic acid, Pentadecanoic acid, and several phenolic derivatives. Conclusion In conclusion, the phytochemicals identified in <ns3:italic>Brassica oleracea</ns3:italic> var. viridis (Collard greens), including Phytol, Omega-3 fatty acids, phenols, flavonoids, and sterols, demonstrate potential benefits for enhancing male fertility and reproductive health. These phytochemicals exert their beneficial action through their antioxidant, anti-inflammatory, and possibly neuroprotective properties. There is need for further research to establish the effectiveness of these phytochemicals in clinical settings and provide concrete guidelines for their use in treating male infertility. </ns3:p>