Comparison of Different Extraction Methods from Ganoderma lucidum IBRC-M 30306 and Evaluating on Multi-resistant Clinical Isolates of Pseudomonas aeruginosa

Some mushrooms have been traditionally used as medicinal components for treating diseases. Ganoderma lucidum contains bioactive components with antibacterial activities. In this research, various techniques were employed to extract terpenoids and polysaccharides from Ganoderma lucidum , and the soxhlet method proved to be the most effective. In addition, the antibacterial effect of the extracted terpenoids and polysaccharides was evaluated against strains such as multi-drug resistant (MDR) Pseudomonas . The results showed that MIC was 0.4-1.7 and 3.75-7.5 mgml -1 , and MBC was 3.43-6.875 and 15-30 mgml -1 for terpenoid and polysaccharide extracts, respectively. In addition, the biofilm formation inhibitory concentrations of polysaccharide extracts against the urinary tract, wound, respiratory system, and standard samples were 0.937, 0.937, 0.46, and 0.23 mgml -1 , respectively. In terms of free radical scavenging activity, the IC50 values for the polysaccharide and terpenoid extracts were 647.76 and 97.194 μgml-1, respectively. Both extracts demonstrated antibacterial properties, with the polysaccharide extract showing stronger antibacterial activity than the terpenoid extract. Terpenoid extract also indicated higher antioxidant properties. The findings revealed that extracts from Ganoderma lucidum may be effective therapeutic agents, particularly against challenging infections like those caused by Pseudomonas.