Geographical Mapping of Liver Fluke (Fasciola hepatica) Prevalence through Snail Sampling in Mayantoc, Tarlac

Abstract— The livestock industry continues to face challenges that hinder growth and productivity, with fasciolosis remaining a significant parasitic disease of concern. Fasciolosis, caused by Fasciola hepatica and Fasciola gigantica, impairs the health, productivity, and reproductive performance of ruminant animals such as cattle, goats, and sheep. This study was conducted to detect Fasciola DNA in snail intermediate hosts and to map potential infestations in goats within selected barangays of Mayantoc, Tarlac. Laboratory techniques employed included DNA extraction, loop-mediated isothermal amplification (LAMP) using Cathepsin B primers, and GIS-based interpolation mapping. Snail samples from selected sites were pulverized, homogenized with extraction reagents, and incubated in LAMP tubes, with positive results indicated by a distinct color change. Surprisingly, no detectable Fasciola DNA was identified in snail samples collected from different barangays. This result may be attributed to effective deworming programs, both oral and injectable, initiated by the Department of Agriculture (DA) and Local Government Unit (LGU) of Mayantoc. The study further explored the demographic profile, knowledge, and practices of 35 smallhold ruminant farmers in the municipality. Results revealed reliance on tethering, limited awareness of fasciolosis, and minimal preventive strategies despite ongoing animal health campaigns. GIS interpolation maps highlighted variations in barangay elevations, providing useful insights into the role of topography in parasite transmission. Collectively, findings confirm that Mayantoc remains liver fluke-free, reinforcing its suitability for ruminant production while underscoring the importance of sustained farmer education and surveillance.