Determination of hydrogen peroxide in milk by fluorometric method using chitosan-coumarin film sensor

ABSTRACT: Accurate determination of hydrogen peroxide is of great importance from a public health standpoint. In this work, determination of hydrogen peroxide in milk was performed using chitosan-coumarin film sensor via a fluorometric method. Coumain-3-carboxylic acid (CCA) was used as a probe in the matrix of chitosan. The structures of the films were confirmed by various instrumental analysis including FT-IR, TGA, and SEM. Various parameters such as the amount of CCA, pH, and the effect of catalyst on the response of the sensor were investigated. The results indicated that ultraviolet radiation could improve the response of the sensor. Hydrogen peroxide was determined using this method in the range of 12-200 μM and 0.5-8.0 mM. The calibration plots demonstrated that the fluorescence response of the sensor films was linear over hydrogen peroxide concentrations of 12-200 µM and 500-8000 µM, with a detection limit (LOD) of 3 µM.