Effects of growth regulators and composition of nutrient media on in vitro regeneration of Juniperus sabina L.

In this study we have to establish and optimize a regeneration for economically important Juniperius sabina L. in Mongolia. Plant regeneration were achieved with leaves/shoots explants collected in the summer with the length of 1.5–2.0 cm. Shoot responses on explants depended on nutrient medium types and the concentration of 6-benzilaminopurine (BAP) growth regulator. Highly efficient shoot formation was obtained when either on Murashige and Skoog (MS), Murashige and Skoog medium with Gamborg’s B5 vitamin (MSB5) and Schenk and Hildebrandt Basal (SH) medium supplemented with 0–1 mg/l 6-benzilaminopurine (BAP) with the length of 0.5–2.6 cm. The highest root induction rate was 75% on SH medium supplemented with 0.5 mg/l naphthalene acetic acid (NAA). The longest root length observed Schenk and Hildebrandt Basal (SH) medium without growth regulator.