Development and Validation of UV Spectroscopic Method for Simultaneous Estimation of Metoprolol Succinate and Cilostazol

In the present work, A Simple, rapid, sensitive, precise, and reproducible, specific UV spectrophotometric method for the determination of Metoprolol succinate and Cilostazol in bulk drug form were developed and validated. The estimated wavelength for Metoprolol succinate was 223nm, while Cilostazol was 257nm. Beer's law applies to Metoprolol succinate and Cilostazol at concentrations of 15-40μg/ml and 6-16μg/ml, respectively with correlation coefficients of 0.9971 and 0.9995. The recovery rates for Metoprolol succinate and Cilostazol were found to be 99.24 - 100.6% and 99.35 - 100.4%, respectively. Intraday precision of Metoprolol succinate and Cilostazol were found to be 0.167 - 0.258 and 0.657 - 0.847 %RSD, while interday precision were found to be 0.118 - 0.287 and 0.426 - 0.747 %RSD respectively. The LOD and LOQ of Metoprolol succinate were found to be 5.971μg/ml and 18.09μg/ml, Cilostazol was found to be 1.075μg/ml and 3.258μg/ml, respectively. Validation of proposed methods was carried out according to ICH Q2R1Guidelines. The proposed methods were found accurate and reproducible for routine analysis of both the drugs.