Verification of the high-sensitivity troponin I assay method on Abbott Alinity I: Experience of the central Biochemistry Laboratory of Ibn Sina University Hospital in Rabat

Verification of an analytical method in clinical laboratories is an essential process under the responsibility of biologists. It consists in assessing the analytical performance of a method and/or technique for assaying a biological parameter, in order to guarantee accurate and reliable results that benefit both the patient and the prescriber. The aim of our work, carried out in the Central Biochemistry Laboratory of the Ibn Sina University Hospital in Rabat-salé, is to verify the Troponin I (cTnI) assay « Range A » on Abbott's Alinity i automated system, using the chemiluminescent microparticle immunoassay (CMIA) method. The methodology adopted was based on the recommendations of the COFRAC (Comité Français d'Accréditation) accreditation technical guide, involving evaluation of the main analytical performances: repeatability, reproducibility and method comparison, plus an external quality assessment (EQA) to measure accuracy. The results obtained from this evaluation were compliant, and the CVs (coefficients of variation) produced were compliant and satisfactory with the supplier's data and with the Ricos learned society.