Design of Aptamer Sandwich Pairs for Colorimetric Detection of TNFa, Interleukin-17A and Dickkopf-1 Proteins Associated with Ankylosing Spondylitis

We employed DNA and 2’-fluoro RNA aptamers to assembly novel systems for colorimetric
\nmicroplate detection of TNFa, interleukin-17A and Dickkopf-1 proteins as biomarkers associated with
\nankylosing spondylitis. For each protein, we chose an optimal sandwich pair made of plate-immobilized
\ncapture aptamer and biotinylated reporter aptamer recruiting streptavidin-peroxidase conjugate. A
\nfeasibility of these test systems was demonstrated in model protein solutions

Publication Details

Journal
SibFU Digital Repository (Siberian Federal University)
Published
2024-10-26
Primary Topic
Spondyloarthritis Studies and Treatments
Type
article
Field-Weighted Citation Impact
0.00
Controls
|||
ALL TIME
JAN
FEB
MAR
APR
MAY
JUN
JUL
AUG
SEP
OCT
article

Design of Aptamer Sandwich Pairs for Colorimetric Detection of TNFa, Interleukin-17A and Dickkopf-1 Proteins Associated with Ankylosing Spondylitis

SibFU Digital Repository (Siberian Federal University)
Spondyloarthritis Studies and Treatments
article

Design of Aptamer Sandwich Pairs for Colorimetric Detection of TNFa, Interleukin-17A and Dickkopf-1 Proteins Associated with Ankylosing Spondylitis

article en

Abstract

We employed DNA and 2’-fluoro RNA aptamers to assembly novel systems for colorimetric
\nmicroplate detection of TNFa, interleukin-17A and Dickkopf-1 proteins as biomarkers associated with
\nankylosing spondylitis. For each protein, we chose an optimal sandwich pair made of plate-immobilized
\ncapture aptamer and biotinylated reporter aptamer recruiting streptavidin-peroxidase conjugate. A
\nfeasibility of these test systems was demonstrated in model protein solutions

SibFU Digital Repository (Siberian Federal University)
Openalex Percentile: Top 41%
Spondyloarthritis Studies and Treatments
Open Access PDFOpenAlex
AI Navigator

Ask Laika to Summarize, Analyze, and Connect papers live on the map.

Summarize Papers & Methodologies

Extract key findings, datasets, and comparative methods across publications.

Benchmark Rankings & Visual Analytics

Rank top research institutions, authors, funders, topics, and journals by Field-Weighted Citation Impact (FWCI) and paper volume with instant charts.

Connect Distant Disciplines

Bridge topological clusters on the map to find hidden collaborative intersections.

Design of Aptamer Sandwich Pairs for Colorimetric Detection of TNFa, Interleukin-17A and Dickkopf-1 Proteins Associated with Ankylosing Spondylitis · SibFU Digital Repository (Siberian Federal University) (2024) | TGRS Research Map | TGRS