UNLOCKING THE POTENTIAL OF ADIPOSE MSCS: NEW ISOLATION TECHNIQUE AND ITS RELEVANCE IN OBESITY THERAPY

Adipose-derived stem cells (ADSCs), were isolated from discarded human fat tissue, obtained from c- section with our recently modified methods, in Stem Cell & Regenerative Medicine Lab, VSBT. Here we develop two methods to isolate Adipose derived mesenchymal stem cells with enzyme digestion and use of phosphatidylcholine and deoxycholate. Surface protein expression was analyzed by flow cytometry to characterize the cell phenotype. The multi-lineage potential of ADSCs was testified by differentiating cells with adipogenic inducer. ADSCs can be cultured in vitro for up to one month without passage. Also, the flow cytometry analysis showed that ADSCs expressed high levels of stem cell related surface marker CD105. ADSCs have strong proliferation ability, maintain their phenotypes, and have stronger multi-differentiation potential. The molecular basis of ADSC differentiation was studied using bioinformatics tools with an aim to identify the key proteins involved in differentiation, such that they could be used as potential targets for drug development for the treatment of obesity. The key proteins involved were found to be PPARG and C/EBPα. The structures of the proteins were retrieved from MMDB (Molecular Modelling Database) and PDB (Protein Data Bank) respectively.