EXPLORING MUMMY'S REGENERATIVE EFFECTS: A COMPARATIVE STUDY ON MSCS FROM WHARTON'S JELLY AND ADIPOSE TISSUE WITH HUMAN FIBROBLASTS

Background: The Wound healing process, as a coordinated physiological mechanism, is a critical subject in medicine. The slow healing and scar formation associated with numerous conventional therapies have led researchers to seek new and more effective therapeutics. This study evaluated the effects of Mummy material, Wharton Jelly Stem Cells (WJSCs), and Adipocyte Stem cells (ASCs) on the fibroblast migration and proliferation. Materials and Methods: It was demonstrated that fibroblast cells could attach to threedimensional (3D) scaffolds in the mentioned microenvironment. ASCs and WJSCs were enriched from human adipose tissue and women undergoing cesarean section, respectively. The proliferation rate, migratio n, expression of fibronectin, collagen I, III, and cell adhesion on PCL scaffold in the presence of mummy material were investigated. Results: The results emphasized the importance of Mummy material, ASCs, and WJSCs in the migration and proliferation of fibroblast cells. The presence of the aforementioned components and cells enhanced the expression of fibronectin (FN1) and collagen types I and III. Additionally, the mummy material was found to promote the proliferation of ADSCs and WJSCs seeded on the PCL scaffold. Together, these findings demonstrate a valuable in vitro technique for studying the healing process. Conclusion: As a result, the potential for using Mummy material and stem cellbased therapeutics in wound healing is exciting.