"EXTRACTION, IDENTIFICATION AND INVITRO ANTIOXIDANT ACTIVITY OF SOME IMPORTANT BIOFLVONOIDS FROM MANGO PEELS" (MANGIFERA INDICA)

Flavonoids are a class of polyphenolic secondary metabolites found with rich contents in colours of fruits flowers and leaves of plants. Mangiferin is bioflavonoid, a phenolic compound, Xanthone glycoside found primarily in mangoes (Mangifera indica). The Fruits pulp peels and, seeds, leaves, and bark of mango tree, has demonstrated potent anti-oxidant, anti- inflammatory, and anti- diabetic properties. In this study we have reported a simple method of extraction, isolation and antioxidant potential of Mangiferin from mango peels. For the extraction of mangiferin we used water and acetone mixture (water / Acetone 30/70: v/v) as a solvents, the % yield was 68.03%. TLC was done to identify Mangiferin in the extract using Ethyl acetate: Methanol: Water: 7:1:2 as mobile phase. The Rf value of Mangiferin was found to be 0.72. Further, Nitric oxide (NO) reducing power method was employed for invitro Antioxidant activity using DMF as a solvent at different concentrations, viz. 20, 40, 60, and 80 µg/ml and ascorbic acid was used as standard drug. The results of the study suggested that Mangiferine revealed the peak at 546.0 nm in DMF, The investigation of invitro antioxidant activity data revealed that Mangiferin by nitric oxide (NO) method showed moderate antioxidant activity compared with standard ascorbic acid.