A Review on Determination of Atropine and Scopolamine by RP-HPLC

A reverse phase high- performance liquid chromatography (HPLC) equipped with a UV-PDA detector was used for the analysis of the main tropane alkaloids, which were found in the roots, aerial parts and seeds of the Pojark plants. Scopolamine was the main alkaloid of the two populations. The populations from Ashtian mountain and Zangane village had the highest and lowest tropane alkaloids content. The alkaloid content of seeds was higher than other parts. The linearity of the method was between 4 and 400 g/ml for atropine and between 0. 8 and 80 g/ml for scopolamine. Limit of detection was 5. 15 and 17. 4 g/ml, and limit of quantification was 1. 92 and 6. 4 g/ml