DETERMINATIONOFCAFFEINECONTENTIN COFFEE BEANS GROWN IN BORENA MEKANE SELAM, ETHIOPIA

<strong>ABSTRACT</strong>: Coffee is an important commodity culturally, commercially and economically in the world. Because of its consumption in most countries in the world, it is important to determine the concentration of caffeine in coffee sample. In this research 6705 UV/vis spectrophotometer was used for the determination of caffeine content in roasted coffee and chloroform was used as a solvent for the coffee sample taken from south Wollo zone Borena wereda in three kebeles .The results show that the caffeine content of Jimay, Gatira and Meskabe kebeles samples were 564,638.4and 668.4 ppm respectively. That is, the caffeine content of Meskabe &gt;Jimay &gt;Gatira roasted samples of coffee. Finally, the results of present study was compared with the caffeine contents of the world’s largest coffee producing countries such as Kenya, Indonesia, Colombia, Vietnam, and Brazil with the range of caffeine content of coffee producing countries.