GREEN SYNTHESIS OF ZINC OXIDE NANOPARTICLES BY USING DATURA STRAMONIUM LEAF EXTRACT FOR ANTIBACTERIAL TREATMENT

Abstract Green synthesis of NPs using different types of extract is a novel method to develop environmentally benign nanomaterials which can be used in numerous biomedical applications. This work provides and addressed a low cost, non-toxic green synthesis of zinc oxide nanoparticles prepared using Datura Stramonium leaf extract as a green alternative template. In this study, ZnO NPs were synthesized using 0.5M zinc acetate dihydrate in the presence of Datura stramonium leaf extract within three volume ratios,7:3 (70mL zinc acetate dihydrate solution and 30mL leaf extract), 4:1 (40mL zinc acietat edihydrate solution and 10 mL Datura stramonium leaf extract), and 9:1 (90mL zinc acetate dihydrate solution and 10 mL Datura stramonium leaf extract). The synthesized nanoparticles were characterized using X-Ray diffraction, scanning electron microscopy, Fourier transform infrared spectroscopy and ultraviolet visible spectroscopy. The XRD analysis showed that ZnO NPs were highly crystalline, pure and the average crystalline size were in the range of (17.92-22.96) nm. The FTIR analysis shows the interaction between the capping agents and synthesized ZnO nanoparticle bond stretching vibration. The UV-Vis analysis proves the band gap energy were 3.34ev, 3.27ev, and 3.25eV for the 4:1, 7:3, and 9:1 volume ratios, respectively. SEM image indicates surface morphology and among the three ratios, 𝑍𝑛𝑂 (4:1) found to be more homogenious.The Antibacterial activity for the (7:3, 4:1, and 9:1) were investigated using Staphylococcus aureus and Escherichia coli within three concentrations of 50, 75 and 100 µg/mL. The antibacterial activity of 𝑍𝑛𝑂 NPs increase with increasing the concentration of ZnO NPs.