Evaluation of DNA and Histological changes in the liver of Albino Rats treated with Silver Nanoparticles

Silver nanoparticles (AgNPs) are widely used in industrial and medical applications. However, there is a growing concernabout the potentialities of AgNPs to induce genotoxicity and DNA damage in humans. Mutations in mitochondrial DNAcause a number of neurological diseases with defined neuropathology; however, mutations in this genome have also beenfound to be important in a number of more common neurodegenerative diseases. In this study, we discuss the importanceof mitochondrial DNA mutations and speculate how such mutations could lead to cell loss. The present study aimed toindicate the harmful toxic effects caused by acrylamide (ACR) on mitochondrial gene from liver of rats and the possibleprotective role of silver nanoparticles derived from Moringa oliefera leaves against toxic effects of ACR. Thirty adult malealbino rats were used for three weeks and randomly divided into six groups. Partial sequences for the mitochondrial COIgene in the six groups were determined. Direct sequencing shows some changes in DNA sequence for mutated samplesin comparison with the negative control group using forward and reverse primers. The treatment of acrylamide-injured ratswith silver nanoparticles of Moringa oleifera ameliorate the histopathological changes induced by acrylamide.