Research data for article: Response of Primary Human Adipocytes to Fatty Acid Treatment

This research aimed to mimic obesity conditions using human adipocytes fattened by incubation with three different types of fatty acids (FAs). This allows us to generate a model of human hypertrophic adipocytes directly comparable to normal adipose cells. Next, we evaluated the phenotypic and functional differences between control and incubated with FAs adipocytes. Achieved results have shown, that adipocytes treated with fatty acids exhibited a greater number of both “large” and “small” lipid droplets, and an increase in lipid droplet formation and maintenance-related proteins, elevated expression of genes encoding proteins involved in the transport of fatty acids, raised secretion of cholesterol and glutamate. Fattening has also resulted in changes in the phenotype of vimentin filaments and actin cytoskeleton reorganization. Finally, it has also been observed that hypertrophy of adipocytes was accompanied by modifications in cell metabolism, phenotypic and quantitative changes in mitochondria with a simultaneous downregulation of genes involved in mitochondrial fusion. In summary, the human research model that we propose allowed us to demonstrate several adjustments in cells mimicking the obesity state, which may contribute to expanding knowledge about obesity and improving treatment strategies for this disease. Data submitted to the repository are initial, unprocessed experiment results. Individual folders (named after the type of research they concern) contain the output data for all technical repetitions of the experiments performed and a .docx file with a description of the samples and the research method used. Data concerning the Western blotting method are photos of membranes (tiff) with bands present on them corresponding to specific proteins. Data concerning the antibody array are photos of membranes (tiff) with dots present on them corresponding to specific proteins. Data obtained as a result of qPCR analysis are presented in the form of Excel files containing Ct (threshold cycle) values for selected genes expressed by cells at the mRNA level. In the case of cholesterol and glutamate secretion levels, initial data are luminescence measurements obtained from corresponding commercial assays, contained in Excel files. Data concerning the ATP rate assay are Excel files with all measurements performed. Data concerning immunocytochemistry and confocal microscopy analysis are photos of cells (tiff) and Excel files with quantitative data.