UV-SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF BUDESONIDE IN BULK AND PHARMACEUTICAL DOSAGE FORM

A new, simple, accurate, and precise first-order derivative spectroscopic method was developed and validated to estimate Budesonide in bulk and Tablet dosage form. The stock solution was prepared by weighing 100 mg of standard Budesonide in a100 ml volumetric flask with Acetonitrile. The stock solution was made to produce 1000 µg/ml with Acetonitrile. Further dilutions were prepared as per the procedure. The drug solution showed the maximum absorbance at 241 nm. The linearity was found in the concentration range of 2-12µg/ml. The correlation coefficient was found to be 0.9982. The regression equation was found to be Y=0.0318x+0.0029. The method was validated for linearity, accuracy, precision, ruggedness, robustness, LOD and LOQ. The LOD and LOQ for estimation of Budesonide were found to be 0.3009 µg/ml and 0.9119 µg/ml respectively. Recovery of Budesonide was found to be in the range of 96-131%. The proposed method was successfully applied for the quantitative determination of Budesonide in bulk and Tablet dosage forms.