Evaluation of the Endoscopic Ultrasound Aspiration Needle (EUS-AN) for Tissue Sampling in a Simplified Laboratory Setting: Performance, Durability, and Potential Applications

Endoscopic ultrasound (EUS)-guided needle aspiration has become a cornerstone technique for obtaining tissue samples in the diagnosis of gastrointestinal tumors. The Endoscopic Ultrasound Aspiration Needle (EUS-AN) is widely utilized in clinical settings with ultrasound guidance. However, its performance and practicality in non-clinical environments—such as training laboratories and research settings where imaging modalities may not be available—have not been thoroughly investigated. This study aimed to evaluate the usability, efficiency, and structural integrity of the EUS-AN in a simplified laboratory setting using real tissue models, without ultrasound guidance. The performance of the EUS-AN was assessed using fresh chicken tissue to simulate submucosal structures. The procedure included controlled needle insertion, vacuum-assisted aspiration, and evaluation of sample quality. Key components analyzed were the sharp bevel tip, adjustable sheath, and vacuum-assisted syringe system. Parameters such as penetration efficiency, tissue aspiration, sample integrity, and needle durability were systematically measured over multiple use cycles. The EUS-AN demonstrated smooth and consistent tissue penetration with minimal resistance. Vacuum-assisted aspiration yielded intact samples without fragmentation, indicating preserved tissue architecture. Post-procedural inspection revealed no structural damage, bending, or clogging of the needle after repeated use. The entire sampling procedure was completed efficiently, confirming its ease of use and practicality in a non-clinical setting. The EUS-AN proved to be a reliable and efficient tool for tissue sampling outside traditional clinical environments. Its robust design and high-performance features support its potential application in research, educational training, and resource-limited settings, thereby broadening its utility beyond conventional EUS-guided procedures. Keywords: Endoscopic Ultrasound Aspiration Needle (EUS-AN), Tissue Sampling, Non-clinical Settings, Vacuum-assisted Aspiration, Submucosal Tissue Model, Needle Durability and Performance