In Vitro Applications of the Biosynthesized Nickel Oxide Nanoparticles from Safflower

The present study aimed to prepare nickel oxide nanoparticles using readily available, inexpensive, and environmentally friendly materials, so this work was done using the extract of the petals of safflower (Carthamus tinctorius L.) to obtain NiO-NPs. The work involved the utilization of two different genotypes of safflower [Dincer 5-18-1 (red flower) and Remzibey-05 (orange flower)] to experiment with which one worked best. The studying of structure, shape and particles size was determined by (FTIR, UV-Vis., XRD and TEM) analysis and all of the acquired data revealed that the nanoparticles were effectively formed. Nickel oxide particles were evaluated biologically in comparison to its precursor of hydroxide Ni(OH)2 under three dilution series (25%, 50%, and 75%) against some microbes [Candida albicans, Staphylococcus aureus, and Escherichia coli]. The results show that the prepared oxides are less effective than their hydroxides, and in general, all compounds show the inhibition effect of microorganism growth at high concentrations.