The Relation Among Serum Insulin Level and Some Biochemical Parameters in Both Diabetes Type I and Type II Patients

The study evaluates the serum level of Malondialdelyde (MDA), Glutathione (GSH), blood glucose, body mass index (BMI), interlukin-33 (IL-33) in both diabetes type 1 and type 2 patients and to determine their correlation between insulin and other parameters. This study has investigated 90 blood samples for both sexes, were collected from patients with diabetes and divided into (35) blood samples from patients with type 1 diabetes (T1DM) their ages (7-40) years, (35) from patients with type 2 diabetes (T2DM) their ages (40-70) years, and (20) blood samples from healthy individuals, and their ages were comparable to that of the patients with diabetes. Samples were collected from Balad General Hospital, Al-Dhuluiyah, the city of Al-Dhuluiyah, and some outpatient medical clinics, for the period from October 2022 until February 2023. parameters which included: Insulin, interleukin -33 that measured by an Enzyme-Linked Immunosorbent Assay (ELISA) while MDA, GSH and glucose measured by Spectrophotometer. The results of the current study showed increase the level of MDA in the serum of patients with T1DM (0.15340±0.06280) μmol/L and T2DM (0.146±0.045) μmol/L in compared with control at (P<0.05). while a significant decrease is in insulin level for both T1DM patients (2.24±0.78) mu/l and T2DM patients (3.93±0.65) mu/l compared to the control group at (P<0.05), IL-33, where a significant increase in T2DM (19.09±2.74) pg/ml compared to T1DM and control (17.49±2.76, 16.06±2.43) pg/ml. Furthermore, decrease GSH in both T1DM and T2DM. While increase level of glucose in patients with T1DM, T2DM (173.06±23.10,140.63±23.27) mgldl respectively in compared to the control (87.13±6.16) mgldl. The study concluded an increase level MDA and glucose while decrease level of insulin and glutathione in both T2DM and T1DM. in addition increase Il-33 and BMI in T2DM, but decrease BMI in T1DM