RSV Pseudovirus Neutralization Assay v1
Here, we develop an experimental system for measuring neutralization titers against RSV F using pseudotyped lentiviral particles. This system is easily adaptable to evaluate neutralization of relevant strains. This method is first described in the manuscript: SimonichCAL,McMahonTE,JuX,YuTC,BrunetteN,Stevens-AyersT,BoeckhMJ,KingNP,GreningerAL,BloomJD.2025.RSV F evolution escapes some monoclonal antibodies but does not strongly erode neutralization by human polyclonal sera. J Virol99:e00531-25. https://doi.org/10.1128/jvi.00531-25
Publication Details
- Published
- 2025-09-24
- DOI
- https://doi.org/10.17504/protocols.io.ewov115yyvr2/v1
- Primary Topic
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Science Foundation
- Bill and Melinda Gates Foundation
- National Institutes of Health
- National Institute of Allergy and Infectious Diseases
- National Institute of Child Health and Human Development
- Eunice Kennedy Shriver National Institute of Child Health and Human Development