Inactivation of Bacteria Using Light-Activated Flavin Mononucleotide

Source: Cheng, C., et al. Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate. J. Vis. Exp. (2022)This video demonstrates a method for evaluating the phototoxic effects of flavin mononucleotide (FMN) on pathogenic bacteria. Bacterial cultures are exposed to increasing concentrations of FMN and illuminated with visible light to generate reactive oxygen species (ROS) that damage cellular components. The surviving bacteria are then quantified to assess the dose-dependent reduction in viability.