Monitoring Bacterial Infection and Effector Protein Delivery in Plant Cells
Source: Lee, H. et. al., Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection. J. Vis. Exp. (2018)This video demonstrates a method for monitoring bacterial infection and effector protein delivery in plant cells using split superfolder green fluorescent protein (sfGFP) complementation combined with confocal microscopy. The approach enables precise visualization of effector translocation into the host cell cytosol.
Publication Details
- Journal
- Journal of Visualized Experiments
- Published
- 2025-09-26
- DOI
- https://doi.org/10.3791/24012
- Primary Topic
- Fungal and yeast genetics research
- Type
- article
- Field-Weighted Citation Impact
- 0.00