Monitoring Bacterial Infection and Effector Protein Delivery in Plant Cells

Source: Lee, H. et. al., Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection. J. Vis. Exp. (2018)This video demonstrates a method for monitoring bacterial infection and effector protein delivery in plant cells using split superfolder green fluorescent protein (sfGFP) complementation combined with confocal microscopy. The approach enables precise visualization of effector translocation into the host cell cytosol.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-09-26
DOI
https://doi.org/10.3791/24012
Primary Topic
Fungal and yeast genetics research
Type
article
Field-Weighted Citation Impact
0.00
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article

Monitoring Bacterial Infection and Effector Protein Delivery in Plant Cells

Journal of Visualized Experiments
Fungal and yeast genetics research
article

Monitoring Bacterial Infection and Effector Protein Delivery in Plant Cells

article en

Abstract

Source: Lee, H. et. al., Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection. J. Vis. Exp. (2018)This video demonstrates a method for monitoring bacterial infection and effector protein delivery in plant cells using split superfolder green fluorescent protein (sfGFP) complementation combined with confocal microscopy. The approach enables precise visualization of effector translocation into the host cell cytosol.

Journal of Visualized Experiments
Zero hunger
Openalex Percentile: Top 47%
Fungal and yeast genetics research
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Monitoring Bacterial Infection and Effector Protein Delivery in Plant Cells · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS