Recombinant Protein Production and Recovery from Bacteria-Derived Vesicles

Source: Streather, B. R., et al. Optimized Production and Analysis of Recombinant Protein-Filled Vesicles from E. coli. J. Vis. Exp. (2023)This video demonstrates the production and isolation of recombinant proteins tagged with Vesicle Nucleating Peptide (VNp) from bacterial vesicles. An inducer triggers VNp-tagged protein expression, driving vesicle formation and encapsulation, followed by centrifugation and sonication for efficient protein recovery without cell lysis

Publication Details

Journal: Journal of Visualized Experiments
Published: 2025-09-26
DOI: https://doi.org/10.3791/24094
Primary Topic: Geodetic Measurements and Engineering Structures
Type: article
Field-Weighted Citation Impact: 0.00

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article

Recombinant Protein Production and Recovery from Bacteria-Derived Vesicles

Sep 26, 2025

Journal of Visualized Experiments

Geodetic Measurements and Engineering Structures

article

Recombinant Protein Production and Recovery from Bacteria-Derived Vesicles

September 26, 2025article en

Abstract

Journal of Visualized Experiments

Zero hunger

Openalex Percentile: Top 49%

Geodetic Measurements and Engineering Structures

DOI Source OpenAlex

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Recombinant Protein Production and Recovery from Bacteria-Derived Vesicles · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS