Isolation of Gram-Negative Bacteria with Genomic Transposon Insertions

Source: Kazi, M. I., et. al., Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing. J. Vis. Exp. (2020)This video demonstrates the selection, quantification, and preservation of Gram-negative recipient bacteria carrying transposon insertions based on kanamycin resistance.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-09-26
DOI
https://doi.org/10.3791/24068
Primary Topic
Cell Image Analysis Techniques
Type
article
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article

Isolation of Gram-Negative Bacteria with Genomic Transposon Insertions

Journal of Visualized Experiments
Cell Image Analysis Techniques
article

Isolation of Gram-Negative Bacteria with Genomic Transposon Insertions

article en

Abstract

Source: Kazi, M. I., et. al., Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing. J. Vis. Exp. (2020)This video demonstrates the selection, quantification, and preservation of Gram-negative recipient bacteria carrying transposon insertions based on kanamycin resistance.

Journal of Visualized Experiments
Zero hunger
Openalex Percentile: Top 35%
Cell Image Analysis Techniques
DOISourceOpenAlex
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Isolation of Gram-Negative Bacteria with Genomic Transposon Insertions · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS