Isolation and Culture of <i>Clostridioides difficile</i> Bacteria from Larval Zebrafish Intestine

Source: Li, J., et. al., Development of a Larval Zebrafish Infection Model for Clostridioides difficile. J. Vis. Exp. (2020)This video demonstrates the isolation and culturing of Clostridioides difficile bacteria from the intestine of larval zebrafish. The infected intestine is dissected, homogenized, and incubated in selective anaerobic media containing antibiotics. These conditions suppress competing microbes and promote the growth of viable cells.