Quantifying Bacterial Alginate Using an Antibody-Based Enzyme-Linked Immunosorbent Assay

Source: Al Ahmar, R., et al. Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate. J. Vis. Exp. (2020)This video demonstrates a colorimetric immunoassay to quantify alginate, a protective exopolysaccharide produced by Pseudomonas aeruginosa. The procedure involves coating a microplate with the sample, applying specific antibodies, and using an enzyme-substrate reaction to generate a measurable color change. A standard curve is then used to determine the alginate concentration based on the sample’s color intensity.