Quantification of Light-Induced Bacterial Inactivation Through Reactive Oxygen Species Generation

Source: Cheng, C., et al. Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate. J. Vis. Exp. (2022).In this video, a buffered reaction mixture containing L-methionine, nitro blue tetrazolium (NBT), and flavin mononucleotide (FMN) is irradiated with violet light to induce reactive oxygen species (ROS) production. This leads to bacterial inactivation and the formation of spectrometrically detectable formazan.