EVALUATION OF ANTIDIABETIC ACTIVITY OF SALVIA NEMOROSA PLANT USING ALPHA AMYLASE INHIBITORY ACTIVITY

The objective of this study was to evaluate the alpha-amylase inhibitory activity of the methanol extract of Salvia nemorosa against porcine pancreatic amylase in vitro. The plant, sourced from a city in Bihar, was extracted using methanol through maceration for 48 hours. Different concentrations (5, 10, 25, and 50 μg/ml) of the methanol extract were prepared and subjected to alpha-amylase inhibitory assay using starch azure with 0.5 M Tris-HCl buffer and 0.01 M CaCl₂ as the substrate. The methanol extract showed significant alpha-amylase inhibitory activity, with an IC₅₀ value of 4.4 μg/ml. The inhibition increased with dose, and when compared with acarbose, a standard drug for the treatment of diabetes, Salvia nemorosa exhibited greater inhibitory potential. These findings suggest that Salvia nemorosa could be a promising candidate for diabetes management; however, further in vivo studies and clinical trials are needed to confirm its therapeutic potential.