Culturing and Maintenance of RNAi-Expressing Bacteria

Source: Anderson, Q. L., et al., A High-throughput, High-content, Liquid-based C. elegans Pathosystem. J. Vis. Exp. (2018)This video demonstrates the preparation of RNA interference (RNAi) bacteria carrying plasmids to inhibit target gene expression. It outlines RNAi bacterial selection, growth, and induction steps to produce dsRNA-expressing plates for downstream assays.

Publication Details

Journal: Journal of Visualized Experiments
Published: 2025-09-26
DOI: https://doi.org/10.3791/24087
Primary Topic: Genomics and Phylogenetic Studies
Type: article
Field-Weighted Citation Impact: 0.00

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article

Culturing and Maintenance of RNAi-Expressing Bacteria

Sep 26, 2025

Journal of Visualized Experiments

Genomics and Phylogenetic Studies

article

Culturing and Maintenance of RNAi-Expressing Bacteria

September 26, 2025article en

Abstract

Journal of Visualized Experiments

Zero hunger

Openalex Percentile: Top 47%

Genomics and Phylogenetic Studies

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Culturing and Maintenance of RNAi-Expressing Bacteria · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS