Evaluation of Post-Surgical Inflammatory and Oxidative Responses in Dogs Undergoing Ovariohysterectomy after Oral Administration of Selenium and Selenium Nanoparticles

Evaluation of oxidative stress status after surgery can be considered as an indicator of any successful treatment after surgery. Therefore, this study investigated the effect of oral administration of selenium and selenium nanoparticles (SeNPs) on surgical oxidative stress status in dogs undergoing ovariohysterectomy (OHE). Twelve female puppies were divided into three equal control, selenium, and nanoselenium groups. In the selenium and nanoselenium groups, capsules containing 50 micrograms of selenium and SeNPs were administered two hours before surgery and followed daily for 4 days after OHE. A significant decrease in SOD activity was observed after OHE compared to before surgery in the control group. While in the nanoselenium group, there was no significant decrease in SOD activity after OHE compared to before surgery. An increase in the activity of SOD, GPx and catalase was observed in the selenium and nanoselenium groups compared to the control group. The lowest MDA concentration was observed in the nanoselenium group. Additionally, high CRP concentrations were observed at 18 and 30 hours after OHE in the control group compared to the two other groups. The increase in the activity of the antioxidant enzymes and the decrease in the serum concentration of MDA and CRP following the oral administration of selenium and SeNPs in dogs that underwent OHE surgery suggest the antioxidant and anti-inflammatory effects of these supplemental treatments after OHE.