Isolation and screening of producing antibacterial compounds bacteria from the salt flats of Bardascan against Drug-Resistant pathogens

Introduction: Diseases caused by drug-resistant bacteria such as methicillin-resistant Staphylococcus aureus are increasing in many medical centers around the world, and researchers are looking for new natural medicinal compounds to overcome and treat these bacteria. Therefore, the current study was conducted with the aim of investigating the antimicrobial effects of native Iranian salt desert bacteria from the environment with difficult growth conditions. Materials & Methods: The isolates were separated and examined for morphology, followed by basic biochemical tests for bacterial identification. Antibacterial activity was assessed in two stages: primary screening via cross-culture and antimicrobial tests on selected active strain extracts using the disk diffusion method. Tests were repeated three times, and growth halo diameters were measured. Finally, the antimicrobial substance production of the selected strain was quantified against MRSA using the disk diffusion method. Results: The study isolated 30 strains from Bardaskan salt flats, with 4 identified as active isolates. One strain, N2, demonstrated strong antimicrobial activity against methicillin-resistant Staphylococcus aureus, with activity increasing with concentration. Maximum antimicrobial substance production occurred 54 hours into fermentation, creating a 29 mm non-growth halo. Data analysis was conducted using Microsoft Excel V.2020. Conclusion: Considering the strong antimicrobial activity of the bacterial extract isolated from the salt Bardaskan against methicillin-resistant Staphylococcus aureus by producing, purifying, and identifying the active compounds of these isolates on an industrial scale, the possibility of introducing new antimicrobial compounds is raised.