Image Resolution Enhancement Algorithm for Different Pairs of Optical Microscope Lenses

In the visible range, the maximum resolution of modern optical microscopes is limited by the diffraction limit. To study objects of smaller sizes, other types of microscopy are used (but these are rather complicated and expensive systems) or methods of program image processing. This paper discusses a program processing method using synthesized aperture techniques. Based on it, the paper shows an experimental method for obtaining high-resolution images from low-resolution images. The images are obtained using optical microscope lenses with different resolutions (10X, 40X and 100X). As a result of processing the obtained images, aperture functions are calculated for each of the considered pairs of lenses. With the help of these aperture functions it is further possible to obtain high-resolution images from low-resolution images, without the use of expensive equipment, or more difficult to operate lenses (e.g., immersion lenses). The developed method will overcome the diffraction limit of optical microscopy, and achieve a resolution that is determined by a given spatial shift. Thus, the quality of optical microscopy measurements will become commensurate with other, more expensive and high-precision types of microscopy, while remaining more accessible and cheaper.