Plant extraction for LC-MS Analysis v1

This protocol describes a rapid and efficient method for extracting metabolites from lyophilized plant material for LC-MS analysis. Samples are homogenized, extracted with ethanol–water, dried, and reconstituted in water–acetonitrile before final centrifugation and transfer to LC-MS vials. The method is optimized for small-scale use and ensures compatibility with LC-MS workflows.

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Publication Details

Published
2025-06-16
DOI
https://doi.org/10.17504/protocols.io.81wgbkj5qgpk/v1
Primary Topic
Metabolomics and Mass Spectrometry Studies
Type
article
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article

Plant extraction for LC-MS Analysis v1

Metabolomics and Mass Spectrometry Studies
article

Plant extraction for LC-MS Analysis v1

article en

Abstract

This protocol describes a rapid and efficient method for extracting metabolites from lyophilized plant material for LC-MS analysis. Samples are homogenized, extracted with ethanol–water, dried, and reconstituted in water–acetonitrile before final centrifugation and transfer to LC-MS vials. The method is optimized for small-scale use and ensures compatibility with LC-MS workflows.

International Olive Council (ES)
Clean water and sanitation
Openalex Percentile: Top 49%
Metabolomics and Mass Spectrometry Studies
DOIOpen Access PDFOpenAlex
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Plant extraction for LC-MS Analysis v1 · (2025) | TGRS Research Map | TGRS