Shotgun Metagenomic Sequencing of Bacterial Enrichments on the Oxford Nanopore Platform Using the R10 flow cell and Ligation sequencing Kit v1

This protocol produces multiplexed amplicon libraries from DNA extracted from general and selective bacterial enrichments using Nanopore's Ligation sequencing kit, suitable for sequencing on Oxford Nanopore Technologies‱ (ONT) MinION/Gridion systems using ONT V14 chemistry (SQK-NBD114-96).

Publication Details

Published
2025-06-16
DOI
https://doi.org/10.17504/protocols.io.81wgbkjqqgpk/v1
Primary Topic
Environmental Toxicology and Ecotoxicology
Type
article
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article

Shotgun Metagenomic Sequencing of Bacterial Enrichments on the Oxford Nanopore Platform Using the R10 flow cell and Ligation sequencing Kit v1

Environmental Toxicology and Ecotoxicology
article

Shotgun Metagenomic Sequencing of Bacterial Enrichments on the Oxford Nanopore Platform Using the R10 flow cell and Ligation sequencing Kit v1

article en

Abstract

This protocol produces multiplexed amplicon libraries from DNA extracted from general and selective bacterial enrichments using Nanopore's Ligation sequencing kit, suitable for sequencing on Oxford Nanopore Technologies‱ (ONT) MinION/Gridion systems using ONT V14 chemistry (SQK-NBD114-96).

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Environmental Toxicology and Ecotoxicology
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Shotgun Metagenomic Sequencing of Bacterial Enrichments on the Oxford Nanopore Platform Using the R10 flow cell and Ligation sequencing Kit v1 · (2025) | TGRS Research Map | TGRS