Standardized SPME-GC-MS Protocol for the Detection of Volatile and Semi-Volatile Compounds in Human Serum v1

This protocol describes the standardized method for the extraction and analysis of volatile and semi-volatile compounds in human blood serum using Solid Phase Microextraction (SPME) with direct fiber immersion, followed by Gas Chromatography-Mass Spectrometry (GC-MS). The method is optimized for use with a DVB/CAR/PDMS fiber and a GC-MS system equipped with an HP-5 column. It enables reproducible, non-targeted metabolic profiling for applications in disease biomarker discovery and environmental exposure assessment. The method is optimized for biological samples and can be applied to studies of disease biomarkers, such as Chagas.