A scalable purification strategy for removal of dsRNA byproducts following IVT RNA production

The presence of double-stranded RNA byproducts in in vitro transcription reactions is a significant concern in the production of synthetic mRNA therapeutics due to their potential to trigger unwanted innate immune responses. This article presents the development, validation, and application of a dsRNA-specific affinity resin using affinity ligand technology coupled with a macroporous base bead.