Production of Silver Nanoparticles from Curvularia tuberculata Fungus and Evaluate its Antimicrobial Activity in Laboratory

The creation of huge amounts of proteins, high yields, and low toxicity of the residues make the fungi as reducing agents in the manufacture of silver nanoparticles.Silver nanoparticles are produced from fungi allow for the control of infections. They can use as antimicrobials in health and agriculture. Current study aimed to produce silver nanoparticles from Curvularia tuberculata fungus that was isolated from the soil. C. tuberculata mycelium filtrate had developed in a liquid fermentation medium in glass flasks for 10 days and resolved with silver nitrate salt AgNo3.The biosynthesis procedure was found by watching the color turns yellow to brown or dark brown by using Ultraviolet spectrometry (UV)that showed absorption peaks at wavelengths 420- 430 nm. A scanning electron microscope (SEM) was revealed the nanoparticles in spherical shapes with dimensions 8–100 nm. The spectrum revealed the existence of amide groups and protein residues, as well as carboxyl groups. The chemical groups in charge of the biosynthesis and encapsulation of nanoparticles were also found using the infrared spectrum (IR) for both fungal filtrates and the resulting particles after converting them into powder. The C. tuberculata showed its ability of silver nanoparticles biosynthesis after 42 hours of incubation. The silver particles produced by fungi detected inhibitory activity against the pathogenic bacteria including P. aeruginosa and S. typhi, they also showed scavenging activity against C. albicans yeast. In conclusion: the C. tuberculata fungus showed the ability to produce silver nanoparticles and the possibility of using particles as antibiotics for pathogenic bacteria and yeasts.