THE EFFECT OF HESPERIDEN & DIOSMIN MIXTURE ON GASTRIC ULCERS INDUCED BY INDOMETHACIN IN MICE

Peptic ulcer is a lesion on the stomach lining known as a gastric ulcer or on the duodenum lining referred to as duodenal ulcers. This leads to a disruption of mucosal integrity. Non-Steroidal AntiInflammatory Drugs (NSAIDs) are primarily responsible for most cases of the disease. Flavonoids provide cytoprotective and restorative effects by enhancing protective factors such as mucus and prostaglandins, also by shielding against potentially harmful elements through their antioxidative, anti-inflammatory, and antibacterial activities. Therefore, this study aims to investigate the effect of Hesperidin and Diosmin Mixture (Hes&Dio) on gastric ulcers induced by Indomethacin in mice. Animals were divided into 4 groups (n= 6 in each): group 1 (Control, CMC), group 2 (Indomethacin), group 3 (Famotidine 8.3 mg/kg), group 4 (Hesperidin and Diosmin 615 mg/kg). Drugs were given orally for 15 days, and then gastric ulcers were induced by a single oral dose of Indomethacin (275 mg/kg) in the last day of the experiment. Histological findings revealed that the group of animals that received Indomethacin experienced ulceration of the entire mucosa, while pre-treatment with Hesperidin and Diosmin reduced ulceration and the depth of lesions in comparison to the Indomethacin group. There was no statistically significant difference observed between the Hes&Dio mixture group and the Famotidine group Our study was the only and the first study that evaluated the effectiveness of mixture of two components (Hesperidin and Diosmin) in Indomethacin induced gastric ulcers, in light of many researches that studied the effect of only one component of the mixture.